ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

46.88-3,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 17.71pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I N-Terminal Propeptide (PINP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen I N-Terminal Propeptide (PINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I N-Terminal Propeptide (PINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Item Name

Procollagen I N-Terminal Propeptide

Research Area

Metabolic pathway;Hepatology;Bone metabolism;

Reference

Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten;Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice.;Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research;Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis;