ELISA Kit for Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

37.0-3,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 12.7pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) and unlabeled Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) (Standards or samples) with the pre-coated antibody specific to Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) in the sample.

Alternative Names

Preptin; Proinsulin-Like Growth Factor-II

Item Name

Pro-Insulin Like Growth Factor II E-Peptide

Research Area

Infection immunity;Hepatology;Hormone metabolism;

Reference

Relationships between preptin and osteocalcin in obese, overweight, and normal weight adults;Assessment of human cartilage glycoprotein 39 (YKL-40), preptin, and nitric oxide in adolescent patients with type 1 diabetes and its relation to cardiorenal affection;