ELISA Kit for Potassium Chloride Cotransporters 1 (KCC1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.127ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Chloride Cotransporters 1 (KCC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Chloride Cotransporters 1 (KCC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Chloride Cotransporters 1 (KCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Potassium Chloride Cotransporters 1 (KCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SLC12A4; hKCC1; Solute Carrier Family 12 Member 4,Potassium/Chloride Transporters; Chloride Potassium Symporter 4; Electroneutral potassium-chloride cotransporter 1

Item Name

Potassium Chloride Cotransporters 1

Research Area

Signal transduction;

Reference

MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection;