ELISA Kit for Platelet Derived Growth Factor Subunit B (PDGFB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 6.3pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Subunit B (PDGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Subunit B (PDGFB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

Item Name

Platelet Derived Growth Factor Subunit B

Research Area

Cytokine;

Reference