SKU:ABCC24885
ELISA Kit for Platelet Derived Growth Factor AA (PDGFAA)
ELISA Kit for Platelet Derived Growth Factor AA (PDGFAA)
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Detection range
15.6-1000pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Mus musculus (Mouse)
Sensitivity
The minimum detectable dose of this kit is typically less than 6.3pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor AA (PDGFAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor AA (PDGFAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor AA (PDGFAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor AA (PDGFAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
PDGF-AA
Item Name
Platelet Derived Growth Factor AA