ELISA Kit for Platelet Activating Factor (PAF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

37.0-3,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 14.7pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Platelet Activating Factor (PAF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Platelet Activating Factor (PAF) and unlabeled Platelet Activating Factor (PAF) (Standards or samples) with the pre-coated antibody specific to Platelet Activating Factor (PAF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Platelet Activating Factor (PAF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Platelet Activating Factor (PAF) in the sample.

Alternative Names

PAF-Acether; AGEPC; Acetyl-Glyceryl-Ether-Phosphorylcholine

Item Name

Platelet Activating Factor

Research Area

Metabolic pathway;Infection immunity;Cardiovascular biology;

Reference

Lysophosphatidylcholine Acyltransferase1 Overexpression Promotes Oral Squamous Cell Carcinoma Progression via Enhanced Biosynthesis of Platelet-Activating Factor;Platelet-activating factor contributes to Bacillus anthracis lethal toxin-associated damage.;Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis;Comparing the role of Ginkgolide B and Ginkgolide K on cultured astrocytes exposed to oxygen‑glucose deprivation;Chlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK Pathway;Antifertility effectiveness of a novel polymer matrix composite and its influence on the endometrium in rhesus macaques (Macaca mulatta);