SKU:ABCC24963
ELISA Kit for Plasminogen (Plg)
ELISA Kit for Plasminogen (Plg)
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Detection range
6.25-400ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Bos taurus; Bovine (Cattle)
Sensitivity
The minimum detectable dose of this kit is typically less than 2.61ng/mL
Sample type
Plasma
Assay length
3h
Method
Double-antibody Sandwich
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen (Plg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen (Plg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen (Plg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plasminogen (Plg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
PL; Plasmin; Activation peptide; Angiostatin
Item Name
Plasminogen
Research Area
Signal transduction;Hematology;
Reference
Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season.;The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid …;