SKU:ABCC25148
ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)
ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Detection range
12.5-800pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Rhesus monkey (Simian)
Sensitivity
The minimum detectable dose of this kit is typically less than 5.3pg/mL
Sample type
Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator Inhibitor 1 (PAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plasminogen Activator Inhibitor 1 (PAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor
Item Name
Plasminogen Activator Inhibitor 1
Research Area
Metabolic pathway;Hematology;