ELISA Kit for Pituitary Adenylate Cyclase Activating Peptide (PACAP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

800-500,000fg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 288fg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pituitary Adenylate Cyclase Activating Peptide (PACAP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pituitary Adenylate Cyclase Activating Peptide (PACAP) and unlabeled Pituitary Adenylate Cyclase Activating Peptide (PACAP) (Standards or samples) with the pre-coated antibody specific to Pituitary Adenylate Cyclase Activating Peptide (PACAP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pituitary Adenylate Cyclase Activating Peptide (PACAP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pituitary Adenylate Cyclase Activating Peptide (PACAP) in the sample.

Alternative Names

ADCYAP1; PRP-48; Pituitary Adenylate Cyclase Activating Peptide

Item Name

Pituitary Adenylate Cyclase Activating Peptide

Research Area

Endocrinology;

Reference

Interictal plasma pituitary adenylate cyclase-activating polypeptide levels are decreased in migraineurs but remain unchanged in patients with tension-type headache;