ELISA Kit for Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.054ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide (PIK3Ca) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PI3K; P110α; p110-alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Serine/threonine protein kinase PIK3CA

Item Name

Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide

Research Area

Enzyme & Kinase;

Reference