ELISA Kit for Phosphatidylinositol Trisphosphate (PIP3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.94ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Phosphatidylinositol Trisphosphate (PIP3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Phosphatidylinositol Trisphosphate (PIP3) and unlabeled Phosphatidylinositol Trisphosphate (PIP3) (Standards or samples) with the pre-coated antibody specific to Phosphatidylinositol Trisphosphate (PIP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Phosphatidylinositol Trisphosphate (PIP3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Phosphatidylinositol Trisphosphate (PIP3) in the sample.

Alternative Names

PtdIns(3,4,5)P3

Item Name

Phosphatidylinositol Trisphosphate

Research Area

Reference