ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antibody Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.65ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h, 30min

Method

Competitive Inhibition

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Item Name

Perinuclear Anti-Neutrophil Cytoplasmic Antibody

Research Area

Reference