ELISA Kit for Peptide YY (PYY)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Sus scrofa; Porcine (Pig)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.92pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Peptide YY (PYY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Peptide YY (PYY) and unlabeled Peptide YY (PYY) (Standards or samples) with the pre-coated antibody specific to Peptide YY (PYY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Peptide YY (PYY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Peptide YY (PYY) in the sample.

Alternative Names

PYY1; Peptide tyrosine tyrosine

Item Name

Peptide YY

Research Area

Neuro science;Hepatology;

Reference

Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs;