SKU:ABCC23916
ELISA Kit for Pepsin (PP)
ELISA Kit for Pepsin (PP)
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Detection range
2.47-200ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Rattus norvegicus (Rat)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.90ng/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pepsin (PP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pepsin (PP) and unlabeled Pepsin (PP) (Standards or samples) with the pre-coated antibody specific to Pepsin (PP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pepsin (PP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pepsin (PP) in the sample.
Alternative Names
Item Name
Pepsin
Research Area
Enzyme & Kinase;Metabolic pathway;Tumor immunity;Gastroenterology;