ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.63pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Parathyroid Hormone Related Protein (PTHrP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone Related Protein (PTHrP) and unlabeled Parathyroid Hormone Related Protein (PTHrP) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone Related Protein (PTHrP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone Related Protein (PTHrP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Parathyroid Hormone Related Protein (PTHrP) in the sample.

Alternative Names

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

Item Name

Parathyroid Hormone Related Protein

Research Area

Tumor immunity;Endocrinology;Hormone metabolism;Bone metabolism;

Reference