ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.23-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.52ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxidized Low Density Lipoprotein (OxLDL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxidized Low Density Lipoprotein (OxLDL) and unlabeled Oxidized Low Density Lipoprotein (OxLDL) (Standards or samples) with the pre-coated antibody specific to Oxidized Low Density Lipoprotein (OxLDL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxidized Low Density Lipoprotein (OxLDL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxidized Low Density Lipoprotein (OxLDL) in the sample.

Alternative Names

OL

Item Name

Oxidized Low Density Lipoprotein

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;

Reference

Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release;Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian Indians;Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis;