ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 6.7pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxidized Low Density Lipoprotein (OxLDL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxidized Low Density Lipoprotein (OxLDL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxidized Low Density Lipoprotein (OxLDL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oxidized Low Density Lipoprotein (OxLDL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

OL

Item Name

Oxidized Low Density Lipoprotein

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;

Reference

Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expression;Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- mice;Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL.;Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase;Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques;Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice;Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice;Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice;PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation;Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition;Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation;Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice;Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract;Anti-diabetic properties of different fractions of Korean red ginseng;