ELISA Kit for Noggin (NOG)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.057ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Noggin (NOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Noggin (NOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Noggin (NOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Noggin (NOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)

Item Name

Noggin

Research Area

Signal transduction;Immune molecule;Neuro science;Bone metabolism;

Reference

Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis;Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis;The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.;The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells.;