ELISA Kit for Neurofilament, Light Polypeptide (NEFL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Oryctolagus cuniculus (Rabbit)

Sensitivity

The minimum detectable dose of this kit is typically less than 50.1pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurofilament, Light Polypeptide (NEFL) and unlabeled Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurofilament, Light Polypeptide (NEFL) in the sample.

Alternative Names

CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

Item Name

Neurofilament, Light Polypeptide

Research Area

Neuro science;

Reference