ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 51.1pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and unlabeled N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) (Standards or samples) with the pre-coated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample.

Alternative Names

NT-Pro-BNP; ; N-BNP

Item Name

N-Terminal Pro-Brain Natriuretic Peptide

Research Area

Endocrinology;Cardiovascular biology;

Reference

Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway;Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in rats;Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats;Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure;Qiliqiangxin ameliorates cardiac dysfunction in heart failure rats post myocardial infarction via activation of HIF/VEGF pathway;Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.;Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway;The potential role of inflammation in cryptogenic stroke;