ELISA Kit for Myostatin (MSTN)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.11-90ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.44ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Myostatin (MSTN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Myostatin (MSTN) and unlabeled Myostatin (MSTN) (Standards or samples) with the pre-coated antibody specific to Myostatin (MSTN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Myostatin (MSTN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Myostatin (MSTN) in the sample.

Alternative Names

GDF8; Growth Differentiation Factor 8

Item Name

Myostatin

Research Area

Cytokine;Metabolic pathway;Bone metabolism;

Reference

The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats;Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats;