ELISA Kit for Myosin Light Chain 2 (MYL2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 29pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 2 (MYL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 2 (MYL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 2 (MYL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 2 (MYL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CMH10; MLC2; Myosin,Light Chain 2,Regulatory,Cardiac,Slow; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; Myosin Light Chain 2, Regulatory, Cardiac

Item Name

Myosin Light Chain 2

Research Area

Metabolic pathway;Cardiovascular biology;Bone metabolism;

Reference