ELISA Kit for Myelin Basic Protein (MBP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 85.7pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Myelin Basic Protein (MBP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Myelin Basic Protein (MBP) and unlabeled Myelin Basic Protein (MBP) (Standards or samples) with the pre-coated antibody specific to Myelin Basic Protein (MBP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Myelin Basic Protein (MBP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Myelin Basic Protein (MBP) in the sample.

Alternative Names

Myelin A1 protein; Myelin membrane encephalitogenic protein

Item Name

Myelin Basic Protein

Research Area

Infection immunity;Neuro science;Autoimmunity;

Reference