ELISA Kit for Mucosae Associated Epithelia Chemokine (MEC)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.055ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucosae Associated Epithelia Chemokine (MEC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucosae Associated Epithelia Chemokine (MEC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucosae Associated Epithelia Chemokine (MEC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucosae Associated Epithelia Chemokine (MEC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CCL28; SCYA28; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 28; Chemokine C-C-Motif Ligand 28; Small-inducible cytokine A28

Item Name

Mucosae Associated Epithelia Chemokine

Research Area

Cytokine;Tumor immunity;Infection immunity;Dermatology;

Reference