ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.132ng/mL

Sample type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 1 (Smad1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 1 (Smad1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

BSP1; JV4-1; JV41; MADH1; MADR1; SMAD Family Member 1; Mad-related protein 1; Transforming growth factor-beta-signaling protein 1; Mothers against DPP homolog 1

Item Name

Mothers Against Decapentaplegic Homolog 1

Research Area

Developmental science;

Reference

Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesis;