ELISA Kit for Mitochondrial Ribosomal Protein L18 (MRPL18)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.061ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Ribosomal Protein L18 (MRPL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Ribosomal Protein L18 (MRPL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Ribosomal Protein L18 (MRPL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitochondrial Ribosomal Protein L18 (MRPL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

L18mt; 39S ribosomal protein L18, mitochondrial

Item Name

Mitochondrial Ribosomal Protein L18

Research Area

Signal transduction;

Reference

Diabetes‐mediated promotion of colon mucosa carcinogenesis is associated with mitochondrial dysfunction;