ELISA Kit for Microsomal Triglyceride Transfer Protein (MTTP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.056ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microsomal Triglyceride Transfer Protein (MTTP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microsomal Triglyceride Transfer Protein (MTTP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microsomal Triglyceride Transfer Protein (MTTP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microsomal Triglyceride Transfer Protein (MTTP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

MTP; ABL; Microsomal triglyceride transfer protein large subunit

Item Name

Microsomal Triglyceride Transfer Protein

Research Area

Metabolic pathway;

Reference

27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brain;27-Hydroxycholesterol regulates cholesterol synthesis and transport in C6 glioma cells.;