ELISA Kit for Meprin A Alpha (MEP1a)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

49.4-4,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 19.1pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Meprin A Alpha (MEP1a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Meprin A Alpha (MEP1a) and unlabeled Meprin A Alpha (MEP1a) (Standards or samples) with the pre-coated antibody specific to Meprin A Alpha (MEP1a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample.

Alternative Names

PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

Item Name

Meprin A Alpha

Research Area

Metabolic pathway;

Reference