ELISA Kit for Lysophosphatidic Acid (LPA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 52.7ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to LPA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled LPA analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of LPA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LPA in the sample.

Alternative Names

LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid

Item Name

Lysophosphatidic Acid

Research Area

Reference

Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R;ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy;