ELISA Kit for Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.62-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 6.1pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

XLKD1; CRSBP-1; HAR; Extracellular Link Domain Containing 1; Hyaluronic acid receptor; Cell surface retention sequence-binding protein 1

Item Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Research Area

CD & Adhesion molecule;

Reference