ELISA Kit for Lipopolysaccharide (LPS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.47-30ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.17ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide (LPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipopolysaccharide (LPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipopolysaccharide (LPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipopolysaccharide (LPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin

Item Name

Lipopolysaccharide

Research Area

Reference

Administration of probiotic mixture DM#1 ameliorated 5-fluorouracil-induced intestinal mucositis and dysbiosis in rats;Coadministration of isomalto-oligosaccharides augments metabolic health benefits of cinnamaldehyde in high fat diet fed mice.;Gastric acid suppression promotes alcoholic liver disease by inducing overgrowth of intestinal Enterococcus.;Food combination based on a pre-hispanic Mexican diet decreases metabolic and cognitive abnormalities and gut microbiota dysbiosis caused by a sucrose-enriched high-fat diet in rats;House Dust Mites Induce Production of Endothelin-1 and Matrix Metalloproteinase-9 in Keratinocytes via Proteinase-Activated Receptor-2 Activation;Long‐Term Genistein Consumption Modifies Gut Microbiota, Improving Glucose Metabolism, Metabolic Endotoxaemia and Cognitive Function in Mice Fed a High‐Fat …;Effects of probiotic supplementation on inflammatory biomarkers and uremic toxins in non-dialysis chronic kidney patients: A double-blind, randomized, placebo …;Effect of systemic administration of lipopolysaccharides derived from on gene expression in mice kidney;アトピー性皮膚炎の痒みの発症機序に関する研究;Repression of TXNIP–NLRP3 axis restores intestinal barrier function via inhibition of myeloperoxidase activity and oxidative stress in nonalcoholic steatohepatitis;Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic …;Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease;Lactobacillus plantarum helps to suppress body weight gain, improve serum lipid profile and ameliorate low-grade inflammation in mice administered with glycerol …;Intestinal iNKT Cells Migrate to Liver and Contribute to Hepatocyte Apoptosis During Alcoholic Liver Disease;Influence of poly (I: C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation;Hepatic progenitor cell activation is induced by the depletion of the gut microbiome in mice;Additional Effect of Dietary Fiber in Patients with Type 2 Diabetes Mellitus Using Metformin and Sulfonylurea: An Open-Label, Pilot Trial;Lipopolysaccharide in systemic circulation induces activation of inflammatory response and oxidative stress in cardiorenal syndrome type 1;Intestinal injury and gut permeability in sickle cell disease;Glycerol Monolaurate‐Mediated Attenuation of Metabolic Syndrome is Associated with the Modulation of Gut Microbiota in High Fat diet‐fed Mice;Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice;High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …;The Combination of Ilexhainanoside D and Ilexsaponin A1 Reduces Liver Inflammation and Improves Intestinal Barrier Function in Mice with High-fat Diet-induced …;