ELISA Kit for Lipopolysaccharide (LPS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.15ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lipopolysaccharide (LPS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lipopolysaccharide (LPS) and unlabeled Lipopolysaccharide (LPS) (Standards or samples) with the pre-coated antibody specific to Lipopolysaccharide (LPS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lipopolysaccharide (LPS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lipopolysaccharide (LPS) in the sample.

Alternative Names

LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin

Item Name

Lipopolysaccharide

Research Area

Reference

Nopal (Opuntia ficus indica) protects from metabolic endotoxemia by modifying gut microbiota in obese rats fed high fat/sucrose diet.;Differential Effect of Sucrose and Fructose in Combination with a High Fat Diet on Intestinal Microbiota and Kidney Oxidative Stress;The Role of Intestinal C‐type Regenerating Islet Derived‐3 Lectins for Nonalcoholic Steatohepatitis;Bioactive Food Abates Metabolic and Synaptic Alterations by Modulation of Gut Microbiota in a Mouse Model of Alzheimer's Disease;Megasphaera elsdenii Lactate Degradation Pattern Shifts in Rumen Acidosis Models;Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study;