ELISA Kit for Leukotriene C4 (LTC4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

370.4-30,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 147.4pg/mL

Sample type

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene C4 (LTC4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene C4 (LTC4) and unlabeled Leukotriene C4 (LTC4) (Standards or samples) with the pre-coated antibody specific to Leukotriene C4 (LTC4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample.

Alternative Names

LT-C4

Item Name

Leukotriene C4

Research Area

Metabolic pathway;Infection immunity;

Reference

5-lipoxygenase activation is involved in the mechanisms of chronic hepatic injury in a rat model of chronic aluminum overload exposure;