ELISA Kit for Lactic Acid (LA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

7.41-600ug/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.92ug/mL

Sample type

Serum, plasma and biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lactic Acid (LA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lactic Acid (LA) and unlabeled Lactic Acid (LA) (Standards or samples) with the pre-coated antibody specific to Lactic Acid (LA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lactic Acid (LA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lactic Acid (LA) in the sample.

Alternative Names

Item Name

Lactic Acid

Research Area

Reference

Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats;Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron;The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer;Long-term exercise prevents hepatic steatosis: a novel role of FABP1 in regulation of autophagy-lysosomal machinery;