ELISA Kit for Kynurenic Acid (KYNA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.89ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Kynurenic Acid (KYNA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Kynurenic Acid (KYNA) and unlabeled Kynurenic Acid (KYNA) (Standards or samples) with the pre-coated antibody specific to Kynurenic Acid (KYNA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kynurenic Acid (KYNA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Kynurenic Acid (KYNA) in the sample.

Alternative Names

Kinurenic acid; Kynuronic acid; Kuinurenic acid; Transtorine

Item Name

Kynurenic Acid

Research Area

Reference

Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats;Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway;