ELISA Kit for Kruppel Like Factor 6 (KLF6)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 32pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 6 (KLF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 6 (KLF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kruppel Like Factor 6 (KLF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kruppel Like Factor 6 (KLF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

BCD1; COPEB; CPBP; GBF; PAC1; ST12; ZF9; Core Promoter Element Binding Protein; GC-Rich Binding Factor; B-cell-derived protein 1; Suppressor of tumorigenicity 12 protein

Item Name

Kruppel Like Factor 6

Research Area

Tumor immunity;

Reference