ELISA Kit for Kinectin 1 (KTN1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 30pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinectin 1 (KTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinectin 1 (KTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinectin 1 (KTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinectin 1 (KTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CG1; KNT; Kinesin Receptor; CG-1 antigen; Kinesin receptor

Item Name

Kinectin 1

Research Area

Signal transduction;

Reference