ELISA Kit for Keratin 6C (KRT6C)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 12.7pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6C (KRT6C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 6C (KRT6C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 6C (KRT6C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 6C (KRT6C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

KRT6E; Keratin 6E; Keratin, type II cytoskeletal 6C; Cytokeratin-6C; Type-II keratin Kb12

Item Name

Keratin 6C

Research Area

Tumor immunity;

Reference