ELISA Kit for Keratan Sulfate (KS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

39.06-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 14.68pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Keratan Sulfate (KS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Keratan Sulfate (KS) and unlabeled Keratan Sulfate (KS) (Standards or samples) with the pre-coated antibody specific to Keratan Sulfate (KS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Keratan Sulfate (KS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Keratan Sulfate (KS) in the sample.

Alternative Names

Keratosulfate

Item Name

Keratan Sulfate

Research Area

Metabolic pathway;Bone metabolism;

Reference