ELISA Kit for Ischemia Modified Albumin (IMA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

370.4-30,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 133.7ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ischemia Modified Albumin (IMA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ischemia Modified Albumin (IMA) and unlabeled Ischemia Modified Albumin (IMA) (Standards or samples) with the pre-coated antibody specific to Ischemia Modified Albumin (IMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ischemia Modified Albumin (IMA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ischemia Modified Albumin (IMA) in the sample.

Alternative Names

Item Name

Ischemia Modified Albumin

Research Area

Metabolic pathway;Cardiovascular biology;

Reference

The role of protein oxidation and DNA damage in elderly hypertension;The impact of protein oxidation on sustained and white coat hypertension;