ELISA Kit for Interleukin 17 (IL17)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

62.5-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Chicken (Gallus)

Sensitivity

The minimum detectable dose of this kit is typically less than 26.6pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Interleukin 17 (IL17) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Interleukin 17 (IL17) and unlabeled Interleukin 17 (IL17) (Standards or samples) with the pre-coated antibody specific to Interleukin 17 (IL17). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Interleukin 17 (IL17) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Interleukin 17 (IL17) in the sample.

Alternative Names

IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8

Item Name

Interleukin 17

Research Area

Cytokine;Infection immunity;

Reference

Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.;