ELISA Kit for Interleukin 1 Theta (IL1q)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

7.8-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 3.1pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Theta (IL1q). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Theta (IL1q). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Theta (IL1q), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Theta (IL1q) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

IL1F10; IL38; FKSG75; IL-1HY2; IL-1F10; FIL1-Theta; Interleukin-38; Interleukin-1 Receptor Antagonist FKSG75; Interleukin 1 Family, Member 10

Item Name

Interleukin 1 Theta

Research Area

Signal transduction;

Reference