ELISA Kit for Interleukin 1 Eta (IL1h)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

7.8-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.8pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Eta (IL1h). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Eta (IL1h). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Eta (IL1h), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Eta (IL1h) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

IL1F8; IL36b; FIL1; FIL1-(ETA); FIL1H; IL-1F8; IL-1H2; IL1-ETA; IL1H2; Interleukin 36, Beta; Interleukin-1 Family Member 8; Interleukin-1 homolog 2

Item Name

Interleukin 1 Eta

Research Area

Cytokine;

Reference