ELISA Kit for Insulin Like Growth Factor 1 (IGF1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.94-60ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Ovis aries; Ovine (Sheep)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.35ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

Item Name

Insulin Like Growth Factor 1

Research Area

Tumor immunity;Endocrinology;

Reference