ELISA Kit for Insulin (INS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

61.7-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 25.3pg/mL

Sample type

serum, plasma, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.

Alternative Names

Item Name

Insulin

Research Area

Metabolic pathway;Endocrinology;Hormone metabolism;

Reference

Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway;Polo Like Kinase 1 (Plk1) Expression in Visceral Adipose Tissue of Morbidly Obese Non Diabetic Women;Effect of NUCKS-1 Overexpression on cytokine profiling in obese women with breast cancer;Antioxidative effects of Korean red ginseng in postmenopausal women: A double-blind randomized controlled trial;Taraxacum officinale;IDENTIFICATION OF MODULATORS OF BINDING PROPERTIES OF ANTIBODIES REACTIVE WITH A MEMBER OF THE INSULIN RECEPTOR FAMILY;Low Circulating Vaspin Level is not associated with Insulinemic Status in Impaired Glucose Tolerant Subjects;Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis;High plasma glucagon levels correlate with waist-to-hip ratio, supra-iliac skinfold thickness, deep subcutaneous abdominal and intra-peritoneal adipose tissue …;High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study;High Plasma Glucagon Levels Correlate with Waist-to-Hip Ratio, Suprailiac Skinfold Thickness, and Deep Subcutaneous Abdominal and Intraperitoneal Adipose Tissue Depots in Nonobese Asian Indian Males with Type 2 Diabetes in North India;Relationship between serum leptin concentration and insulin resistance syndrome in patients with type 2 diabetes mellitus;Adipose tissue complement factor B promotes adipocyte maturation.;The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.;Diet enriched with fresh coconut decreases blood glucose levels and body weight in normal adults;Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis;Hyperandrogenism and insulin resistance contribute to hepatic steatosis and inflammation in female rat liver;Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.;Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism;Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training;High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …;