ELISA Kit for Insulin (INS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

61.7-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Sus scrofa; Porcine (Pig)

Sensitivity

The minimum detectable dose of this kit is typically less than 23.5pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.

Alternative Names

Item Name

Insulin

Research Area

Metabolic pathway;Endocrinology;Hormone metabolism;

Reference

Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs.;Targeted folate-conjugated pluronic P85/poly(lactide--glycolide) polymersome for the oral delivery of insulin;Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …;