ELISA Kit for Inositol Triphosphate (IP3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 50.1pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inositol Triphosphate (IP3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inositol Triphosphate (IP3) and unlabeled Inositol Triphosphate (IP3) (Standards or samples) with the pre-coated antibody specific to Inositol Triphosphate (IP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample.

Alternative Names

InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

Item Name

Inositol Triphosphate

Research Area

Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism;

Reference

The association between the expression of PAR2 and TMEM16A and neuropathic pain;OSBP-related protein 4L promotes phospholipase Cβ3 translocation from the nucleus to the plasma membrane in Jurkat T-cells;