ELISA Kit for Inhibitory Subunit Of NF Kappa B Zeta (IkBz)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 34pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitory Subunit Of NF Kappa B Zeta (IkBz). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitory Subunit Of NF Kappa B Zeta (IkBz). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitory Subunit Of NF Kappa B Zeta (IkBz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitory Subunit Of NF Kappa B Zeta (IkBz) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

NFKBIZ; INAP; MAIL; IL-1 inducible nuclear ankyrin-repeat protein; Molecule possessing ankyrin repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta

Item Name

Inhibitory Subunit Of NF Kappa B Zeta

Research Area

Signal transduction;Infection immunity;

Reference