ELISA Kit for Inhibin Beta E (INHbE)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.09-250pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.21pg/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin Beta E (INHbE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin Beta E (INHbE) and unlabeled Inhibin Beta E (INHbE) (Standards or samples) with the pre-coated antibody specific to Inhibin Beta E (INHbE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin Beta E (INHbE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin Beta E (INHbE) in the sample.

Alternative Names

INH-bE; Activin beta-E chain

Item Name

Inhibin Beta E

Research Area

Endocrinology;

Reference

Inhibin βE (INHBE) is a possible insulin resistance-associated hepatokine identified by comprehensive gene expression analysis in human liver biopsy …;Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine;