ELISA Kit for Inhibin B (INHB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.84pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin B (INHB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin B (INHB) and unlabeled Inhibin B (INHB) (Standards or samples) with the pre-coated antibody specific to Inhibin B (INHB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin B (INHB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin B (INHB) in the sample.

Alternative Names

INH-B

Item Name

Inhibin B

Research Area

Reproductive science;Genetic science;Hormone metabolism;

Reference

Overexpression of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) in boys with cryptorchidism;Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian …;Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the …;